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KMID : 0613820190290050555
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Journal of Life Science
2019 Volume.29 No. 5 p.555 ~ p.563
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Protective Effects of an Ethanol Extract of Elaeagnus umbellata Leaves on α-MSH-induced Melanin Production in B16-F0 Cells and UVB-induced Damage in CCD-986sk Cells
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Park Se-Ho
Jhee Kwang-Hwan
Yang Seun-Ah
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Abstract
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This study was undertaken to investigate the effect of an ethanol extract of Elaeagnus umbellata leaves (EUL-EE) on skin-related biological activities. Previously, we have reported that gallic acid was the major phenolic compound in EUL-EE through quantitative analysis and that EUL-EE had an inhibitory effect against the proliferation of liver cancer HepG2 cells. In the present study, the inhibitory effects of EUL-EE on melanin production and tyrosinase activity in α-melanocyte-stimulated hormone-stimulated B16-F0 cells were determined to assess the effects of EUL-EE on skin whitening. The anti-wrinkle effect using UVB-irradiated CCD-986sk cells was examined by the expression of type I procollagen and metalloproteinase (MMP)-1 release. The EUL-EE significantly decreased intracellular melanin production (33.0% inhibition at 100 μg/ml) when compared with untreated B16-F0 cells. Tyrosinase activities in the stimulated B16-F0 cells were also decreased by EUL-EE (47.8% inhibition at 100 μg/ml). The EUL-EE also dose-dependently increased the production of type I procollagen (up to 1.74-fold at 250 μg/ml) in CCD-986sk cells when compared with UVB-irradiated controls. EUL-EE showed no cytotoxicity at concentrations up to 500 μg/ml. In addition, EUL-EE at 10-500 μg/ml inhibited the release of MMP-1 to the medium from UVB-irradiated CCD-986sk cells. Taken together, these observations indicate that EUL-EE has high potential for use as inner beauty and cosmetic materials due to its whitening and anti-wrinkle effects.
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KEYWORD
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Anti-wrinkle, Elaeagnus umbellata leaves, melanin production, reactive oxygen species
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